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cd25 apc clone: bc96 antibody  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cd25 apc clone: bc96 antibody
    Gating strategy for the assessment of peripheral blood regulatory T cells (Treg) by flow cytometry. Panel ( A ) shows representative dot plots illustrating gating strategy, including exclusion of doublets using forward scatter area (FSC-A) versus forward scatter width (FSC-W) analysis ( A - i ), gating on live cells negative for aminereactive fixable viability dye ( A - ii ), lymphocytes ( A - iii ), and CD3 + T cells ( A - iv ). Next, total <t>CD25</t> and Foxp3, expressing T cells among CD4 + CD127 low population, were analyzed (Panel ( B )). ( B - i ) shows representative gating strategy, ( B - ii ) representative samples from LS and HS protocol. FlowLogic software was used for data analysis and illustration.
    Cd25 Apc Clone: Bc96 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd25+apc+clone%3A+bc96+antibody/sandwich+elisa+kit/pmc08944840-114-41-24
    Average 90 stars, based on 1 article reviews
    cd25 apc clone: bc96 antibody - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Role of Oxidative Stress in Vascular Low-Grade Inflammation Initiation Due to Acute Salt Loading in Young Healthy Individuals"

    Article Title: Role of Oxidative Stress in Vascular Low-Grade Inflammation Initiation Due to Acute Salt Loading in Young Healthy Individuals

    Journal: Antioxidants

    doi: 10.3390/antiox11030444

    Gating strategy for the assessment of peripheral blood regulatory T cells (Treg) by flow cytometry. Panel ( A ) shows representative dot plots illustrating gating strategy, including exclusion of doublets using forward scatter area (FSC-A) versus forward scatter width (FSC-W) analysis ( A - i ), gating on live cells negative for aminereactive fixable viability dye ( A - ii ), lymphocytes ( A - iii ), and CD3 + T cells ( A - iv ). Next, total CD25 and Foxp3, expressing T cells among CD4 + CD127 low population, were analyzed (Panel ( B )). ( B - i ) shows representative gating strategy, ( B - ii ) representative samples from LS and HS protocol. FlowLogic software was used for data analysis and illustration.
    Figure Legend Snippet: Gating strategy for the assessment of peripheral blood regulatory T cells (Treg) by flow cytometry. Panel ( A ) shows representative dot plots illustrating gating strategy, including exclusion of doublets using forward scatter area (FSC-A) versus forward scatter width (FSC-W) analysis ( A - i ), gating on live cells negative for aminereactive fixable viability dye ( A - ii ), lymphocytes ( A - iii ), and CD3 + T cells ( A - iv ). Next, total CD25 and Foxp3, expressing T cells among CD4 + CD127 low population, were analyzed (Panel ( B )). ( B - i ) shows representative gating strategy, ( B - ii ) representative samples from LS and HS protocol. FlowLogic software was used for data analysis and illustration.

    Techniques Used: Flow Cytometry, Expressing, Software

    Related Articles

    Expressing:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Activation Assay:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Marker:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Flow Cytometry:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Control:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    In Vitro:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Cell Isolation:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Staining:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Labeling:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Comparison:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Co-Culture Assay:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Cell Culture:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Generated:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    MANN-WHITNEY:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Recombinant:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Selection:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Software:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.

    Recombinase Polymerase Amplification:

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.


    Article Title: Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals
    Article Snippet: APC-efluor780 anti-human CD25 (clone BC96) , Invitrogen , 47-0259-42.

    Article Title: Regulatory T cell epitopes, compositions and uses thereof
    Article Snippet: Following cell surface staining, cells are fixed and permeabilized (eBioscience) and stained intracellulary for FOXp3 (clone PCH101; eBioscience) following manufacturer's protocol.

    Article Title: Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.
    Article Snippet: The contents were incubated with 10 μl anti-human CD4 (FITC, clone OKT4, Catalog # 11-0048-42,eBioscience, USA) and 10 μl anti-human CD25 (APC, clone BC96, Catalog #17-0259-42, eBioscience, USA) for 30 min at room temperature.Later, 2 ml 1×FCM Lysing Solution (Catalog #70- LYS01,Multiscienses, China) was added to each tube and incubated at room temperature for 15 min in the dark.

    Article Title: Enhanced T cell activation and cytotoxicity against AML via targeted anti-CD99 nanoparticle treatment
    Article Snippet: The master mix was prepared using anti-human CD3 FITC (Clone: UCHT1; Biolegend, Cat#300406), Anti-Human CD4 PE (Clone: OKT4; eBioscience Cat#4337556), Anti-Hu CD8a PE-Cyanine 7 (Clone: SK1; eBioscience Cat#2066348) and Anti-Hu CD25 APC (Clone: BC96; eBioscience Cat#1946588) antibodies.

    Article Title: Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosus
    Article Snippet: For the assessment of human Tregs, cells were stained with FITC conjugated anti-human-CD4 (clone# A161A1) and APC conjugated anti-human-CD25 (clone# BC96), followed by fixing and permeabilizing using FoxP3 / Transcription Factor Staining Buffer Set (catlog# 00-5523-00, eBioscience, San Diego, USA).

    Article Title: Single cell profiling of hematopoietic stem cell transplant recipients reveals TGF-β1 and IL-2 confer immunoregulatory functions to NK cells
    Article Snippet: APC anti-human CD25 (clone BC96) , eBioscience , Cat#17-0259-42; RRID: AB_1582219.



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    Thermo Fisher cd25 apc clone: bc96 antibody
    Gating strategy for the assessment of peripheral blood regulatory T cells (Treg) by flow cytometry. Panel ( A ) shows representative dot plots illustrating gating strategy, including exclusion of doublets using forward scatter area (FSC-A) versus forward scatter width (FSC-W) analysis ( A - i ), gating on live cells negative for aminereactive fixable viability dye ( A - ii ), lymphocytes ( A - iii ), and CD3 + T cells ( A - iv ). Next, total <t>CD25</t> and Foxp3, expressing T cells among CD4 + CD127 low population, were analyzed (Panel ( B )). ( B - i ) shows representative gating strategy, ( B - ii ) representative samples from LS and HS protocol. FlowLogic software was used for data analysis and illustration.
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    Image Search Results


    Gating strategy for the assessment of peripheral blood regulatory T cells (Treg) by flow cytometry. Panel ( A ) shows representative dot plots illustrating gating strategy, including exclusion of doublets using forward scatter area (FSC-A) versus forward scatter width (FSC-W) analysis ( A - i ), gating on live cells negative for aminereactive fixable viability dye ( A - ii ), lymphocytes ( A - iii ), and CD3 + T cells ( A - iv ). Next, total CD25 and Foxp3, expressing T cells among CD4 + CD127 low population, were analyzed (Panel ( B )). ( B - i ) shows representative gating strategy, ( B - ii ) representative samples from LS and HS protocol. FlowLogic software was used for data analysis and illustration.

    Journal: Antioxidants

    Article Title: Role of Oxidative Stress in Vascular Low-Grade Inflammation Initiation Due to Acute Salt Loading in Young Healthy Individuals

    doi: 10.3390/antiox11030444

    Figure Lengend Snippet: Gating strategy for the assessment of peripheral blood regulatory T cells (Treg) by flow cytometry. Panel ( A ) shows representative dot plots illustrating gating strategy, including exclusion of doublets using forward scatter area (FSC-A) versus forward scatter width (FSC-W) analysis ( A - i ), gating on live cells negative for aminereactive fixable viability dye ( A - ii ), lymphocytes ( A - iii ), and CD3 + T cells ( A - iv ). Next, total CD25 and Foxp3, expressing T cells among CD4 + CD127 low population, were analyzed (Panel ( B )). ( B - i ) shows representative gating strategy, ( B - ii ) representative samples from LS and HS protocol. FlowLogic software was used for data analysis and illustration.

    Article Snippet: In order to determine expression of Treg specific cell surface antigens, the following antibody mixture was used: CD3 FITC (clone: OKT3, eBioscienceTM, Affymetrix by Thermo Fisher Scientific, CA, USA), CD4 PerCP-eFluorTM 710 (clone: SK3, eBioscienceTM), CD127 PE-Cy7 (clone: eBioRDR5, eBioscienceTM), and CD25 APC (clone: BC96, eBioscienceTM); the Foxp3 PE (clone: 235A/E7, eBioscienceTM) antibody was used for the detection of Treg signature transcription factor Foxp3.

    Techniques: Flow Cytometry, Expressing, Software